DNMT3B Antibody AF7646 from R&D Systems, a Bio-Techne Brand | Biocompare.com

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Nature communications
May 15 2019

Fig 1: SALL3 interacts with DNMT3B and modulates DNMT function. a Lysate prepared from 253G1 cells was immunoprecipitated with anti-SALL3 or normal rabbit IgG. The immunoblot was analyzed with anti-DNMT3A, DNMT3B, and DNMT1 antibodies. Molecular weight is indicated as Mr (k). b Lysate prepared from 253G1 cells was immunoprecipitated with anti-DNMT3B or normal sheep IgG. The immunoblot was analyzed with anti-SALL3 antibody. c In vitro DNMT activity assay. DNMT activity of the nuclear fractions prepared from 253G1 (WT) cells and the 253G1 SALL3-/- cells was measured (n = 3, biological replicates). *P < 0.01, two-sided t test. d qRT-PCR analysis of neural cell markers PAX6 and SOX1. Total RNA was isolated from 253G1-derived (WT), 253G1 SALL3-/--derived and 253G1 SALL3-/- DNMT3B-/mut-derived neural cells (n = 6, biological replicates). *P < 0.01, one-way ANOVA with post hoc Tukey–Kramer test. e qRT-PCR analysis of cardiomyocyte markers GATA4, NKX2.5, and TNNT2. Total RNA was isolated from 253G1-derived (WT), 253G1 SALL3-/--derived and 253G1 SALL3-/- DNMT3B-/mut-derived cardiomyocytes (n = 6, biological replicates). *P < 0.01, one-way ANOVA with post hoc Tukey–Kramer test. Error bars represent mean ± SD

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R&D Systems, a Bio-Techne Brand for
DNMT3B Antibody

R&D Systems, a Bio-Techne Brand's DNMT3B Antibody is a Sheep Polyclonal antibody. This antibody has been shown to work in applications such as: Simple Western, Immunocytochemistry, and Western Blot. The DNMT3B Antibody was generated using DNMT3B as the antigen and it reacts with Homo Sapiens, and Human.

Product Specs
ItemDNMT3B Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAF7646
Quantity100 ug (also 25 ug)
ApplicationsWestern Blot, Simple Western, Immunocytochemistry
Conjugate/TagUnconjugated
ReactivityHuman
TargetDNMT3B
IsotypeIgG
HostSheep
NCBI Full Gene NameDNA methyltransferase 3 beta
NCBI Gene AliasesM.HsaIIIB, ICF, FSHD4, ICF1
ClonalityPolyclonal
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ImmunogenE. coli-derived recombinant human DNMT3B, Ser145-Pro355, Accession # Q9UBC3
ConcentrationLYOPH
PurityAntigen Affinity-purified
Brand/TrademarkR&D Systems, a Bio-Techne Brand
ValidationKnockout Validated
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Specificity: Detects human DNMT3B in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human DNMT3A is observed

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

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